Identification of otubain 1 as a novel substrate for the Yersinia protein kinase using chemical genetics and mass spectrometry.
Yersinia encodes a protein kinase, YpkA, which disrupts the actin cytoskeleton. Using an approach termed chemical genetics, we identified a 36-kDa substrate for YpkA in both J774 lysates and bovine brain cytosol. Mass spectrometry analysis identified this substrate as FLJ20113, an open reading frame that corresponds to otubain 1, a ... deubiquitinating enzyme implicated in immune cell clonal anergy. We demonstrate that otubain 1 is phosphorylated by YpkA in vitro and interacts with YpkA and actin in vivo. Identification of otubain 1 as a YpkA substrate suggests that regulation of immune cell anergy may be a survival mechanism for Yersinia.
Mesh Terms:
Animals, Bacterial Proteins, Brain Chemistry, Cattle, Cell Line, Cell-Free System, Clonal Anergy, Cysteine Endopeptidases, Mice, Phosphorylation, Protein-Serine-Threonine Kinases, Substrate Specificity, Yersinia
Animals, Bacterial Proteins, Brain Chemistry, Cattle, Cell Line, Cell-Free System, Clonal Anergy, Cysteine Endopeptidases, Mice, Phosphorylation, Protein-Serine-Threonine Kinases, Substrate Specificity, Yersinia
FEBS Lett.
Date: Jan. 09, 2006
PubMed ID: 16364312
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