A chromatin remodeling protein is a component of fission yeast mediator.
The multiprotein Mediator complex is an important regulator of RNA polymerase II-dependent genes in eukaryotic cells. In contrast to the situation in many other eukaryotes, the conserved Med15 protein is not a stable component of Mediator isolated from fission yeast. We here demonstrate that Med15 exists in a protein complex ... together with Hrp1, a CHD1 ATP-dependent chromatin remodeling protein. The Med15/Hrp1 subcomplex is not a component of the core Mediator complex, but can interact with the L-Mediator conformation. Deletion of med15+ and hrp1+ cause very similar effects on global steady-state levels of mRNA and genome-wide analyses demonstrate that Med15 associates with a distinct subset of Hrp1-bound gene promoters. Our findings therefore indicate that Mediator may directly influence histone density at regulated promoters.
Mesh Terms:
Cell Cycle Proteins, Green Fluorescent Proteins, Kinetochores, Microtubule-Associated Proteins, Microtubules, Mitotic Spindle Apparatus, Nuclear Proteins, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Cell Cycle Proteins, Green Fluorescent Proteins, Kinetochores, Microtubule-Associated Proteins, Microtubules, Mitotic Spindle Apparatus, Nuclear Proteins, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
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Date: Jul. 09, 2010
PubMed ID: 20622008
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