The Leo1 subunit of yeast Paf1 complex binds RNA and contributes to complex recruitment.
The Paf1 complex (Paf1C) affects RNA polymerase II transcription by coordinating co-transcriptional chromatin modifications and helping recruit mRNA 3' end processing factors. Paf1C crosslinks to transcribed genes, but not downstream of the cleavage and polyadenylation site, suggesting it may interact with the nascent mRNA. Paf1C purified from Saccharomyces cerevisiae binds ... RNA in vitro, as do the purified Leo1 and Rtf1 subunits of the complex. In vivo crosslinking and immunoprecipitation of RNA associated with Paf1C (RNA-IP) shows that Leo1, but not Rtf1, is necessary for the complex to bind RNA. Cells lacking Leo1 have reduced Paf1C recruitment as well as decreased levels of histone H3 and tri-methylated H3K4 within transcribed chromatin. Together, these results suggest that association of Paf1C with RNA stabilizes its localization at actively transcribed regions where it influences chromatin structure.
Mesh Terms:
Amino Acid Sequence, Animals, Basement Membrane, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Movement, Cloning, Molecular, Disintegrins, Extracellular Matrix, Gene Expression Profiling, Genes, Helminth, Glycosylation, Gonads, Metalloendopeptidases, Molecular Sequence Data, Muscles, Mutation, Protein Structure, Tertiary, Recombinant Fusion Proteins, Sequence Alignment, Transgenes
Amino Acid Sequence, Animals, Basement Membrane, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Movement, Cloning, Molecular, Disintegrins, Extracellular Matrix, Gene Expression Profiling, Genes, Helminth, Glycosylation, Gonads, Metalloendopeptidases, Molecular Sequence Data, Muscles, Mutation, Protein Structure, Tertiary, Recombinant Fusion Proteins, Sequence Alignment, Transgenes
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Date: Aug. 23, 2010
PubMed ID: 20732871
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