Self-association of LIM-kinase 1 mediated by the interaction between an N-terminal LIM domain and a C-terminal kinase domain.

LIM-kinase 1 (LIMK1) and 2 (LIMK2) are members of a novel class of protein kinases containing two LIM motifs at the N-terminus. The LIM motif is thought to be involved in protein-protein interactions. We report here evidence that LIMK1 self-associates and also associates with LIMK2. In vivo and in vitro ...
binding analyses using variously deleted mutants of LIMKI revealed that the self-association of LIMK1 was caused by interaction between the N-terminal LIM domain and the C-terminal kinase domain. The association of LIMK1 with itself and with LIMK2 is important for understanding how activities and functions of LIMK family kinases are regulated.
Mesh Terms:
Animals, Base Sequence, COS Cells, DNA Primers, DNA-Binding Proteins, Lim Kinases, Molecular Sequence Data, Protein Binding, Protein Kinases, Protein-Serine-Threonine Kinases
FEBS Lett.
Date: Dec. 09, 1996
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