Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding.
The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is ... essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.
Mesh Terms:
Base Sequence, Binding Sites, Cells, Cultured, Cytoplasm, DNA-Binding Proteins, Endocytosis, Endosomal Sorting Complexes Required for Transport, Exocytosis, Fluorescent Antibody Technique, Gene Library, Gene Products, gag, HIV, Humans, Molecular Sequence Data, Peptide Fragments, Plasmids, Protein Transport, RNA, Small Interfering, Receptor, Epidermal Growth Factor, Sequence Analysis, DNA, Transcription Factors, Transfection, Two-Hybrid System Techniques, Ubiquitin-Protein Ligases, Ubiquitins
Base Sequence, Binding Sites, Cells, Cultured, Cytoplasm, DNA-Binding Proteins, Endocytosis, Endosomal Sorting Complexes Required for Transport, Exocytosis, Fluorescent Antibody Technique, Gene Library, Gene Products, gag, HIV, Humans, Molecular Sequence Data, Peptide Fragments, Plasmids, Protein Transport, RNA, Small Interfering, Receptor, Epidermal Growth Factor, Sequence Analysis, DNA, Transcription Factors, Transfection, Two-Hybrid System Techniques, Ubiquitin-Protein Ligases, Ubiquitins
Genes Dev.
Date: Jul. 15, 2004
PubMed ID: 15256501
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- Interactions 10
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