Activation of Rac1 by a Crk SH3-binding protein, DOCK180.
DOCK180 is involved in integrin signaling through CrkII-p130(Cas) complexes. We have studied the involvement of DOCK180 in Rac1 signaling cascades. DOCK180 activated JNK in a manner dependent on Rac1, Cdc42Hs, and SEK, and overexpression of DOCK180 increased the amount of GTP-bound Rac1 in 293T cells. Coexpression of CrkII and p130(Cas) ... enhanced this DOCK180-dependent activation of Rac1. Furthermore, we observed direct binding of DOCK180 to Rac1, but not to RhoA or Cdc42Hs. Dominant-negative Rac1 suppressed DOCK180-induced membrane spreading. These results strongly suggest that DOCK180 is a novel activator of Rac1 and involved in integrin signaling.
Mesh Terms:
3T3 Cells, Animals, Calcium-Calmodulin-Dependent Protein Kinases, Enzyme Activation, GTP Phosphohydrolases, GTP-Binding Proteins, Integrins, JNK Mitogen-Activated Protein Kinases, Membrane Proteins, Mice, Mitogen-Activated Protein Kinases, Protein Binding, Protein Biosynthesis, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-crk, Signal Transduction, rac GTP-Binding Proteins, src Homology Domains
3T3 Cells, Animals, Calcium-Calmodulin-Dependent Protein Kinases, Enzyme Activation, GTP Phosphohydrolases, GTP-Binding Proteins, Integrins, JNK Mitogen-Activated Protein Kinases, Membrane Proteins, Mice, Mitogen-Activated Protein Kinases, Protein Binding, Protein Biosynthesis, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-crk, Signal Transduction, rac GTP-Binding Proteins, src Homology Domains
Genes Dev.
Date: Nov. 01, 1998
PubMed ID: 9808620
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