Transcriptional and subcellular regulation of the TRIP-Br family.

TRIP-Brs are a recently discovered set of proteins whose functions remain poorly characterized. Here we report the identification of TRIP-Br3 as a member of the TRIP-Br family along with evidence showing that TRIP-Brs interact with bromodomain-containing transcriptional cofactors PCAF, STAF65gamma, and KAP1. PCAF, a histone acetyltransferase; STAF65gamma, a protein associated ...
with histone acetylation activity; and KAP1, a corepressor, influence the transcriptional activity of TRIP-Brs differentially. Finally, while all three TRIP-Brs are localized to the nucleus, TRIP-Br2 and TRIP-Br3 are also present in the cytoplasm through interaction with CRM1. Our results suggest that different TRIP-Brs function by interacting with a wide variety of bromodomain-containing transcriptional regulators in different subcellular locales.
Mesh Terms:
Active Transport, Cell Nucleus, Binding Sites, Blotting, Western, Cell Cycle Proteins, Cell Line, Cell Nucleus, Cytoplasm, DNA-Binding Proteins, Fatty Acids, Unsaturated, Green Fluorescent Proteins, Hela Cells, Histone Acetyltransferases, Humans, Immunoprecipitation, Microscopy, Fluorescence, Nuclear Proteins, Protein Binding, Protein Interaction Mapping, Recombinant Fusion Proteins, Repressor Proteins, Trans-Activators, Transcription Factors, Transcriptional Activation, p300-CBP Transcription Factors
Gene
Date: Feb. 15, 2007
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