The N-terminus of Prp1 (Prp6/U5-102 K) is essential for spliceosome activation in vivo.
The spliceosomal protein Prp1 (Prp6/U5-102 K) is necessary for the integrity of pre-catalytic spliceosomal complexes. We have identified a novel regulatory function for Prp1. Expression of mutations in the N-terminus of Prp1 leads to the accumulation of pre-catalytic spliceosomal complexes containing the five snRNAs U1, U2, U5 and U4/U6 and ... pre-mRNAs. The mutations in the N-terminus, which prevent splicing to occur, include in vitro and in vivo identified phosphorylation sites of Prp4 kinase. These sites are highly conserved in the human ortholog U5-102 K. The results presented here demonstrate that structural integrity of the N-terminus is required to mediate a splicing event, but is not necessary for the assembly of spliceosomes.
Mesh Terms:
Amino Acid Sequence, Molecular Sequence Data, Phosphorylation, Protein-Serine-Threonine Kinases, Ribonucleoprotein, U4-U6 Small Nuclear, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Deletion, Spliceosomes
Amino Acid Sequence, Molecular Sequence Data, Phosphorylation, Protein-Serine-Threonine Kinases, Ribonucleoprotein, U4-U6 Small Nuclear, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Deletion, Spliceosomes
Nucleic Acids Res.
Date: Mar. 01, 2010
PubMed ID: 20007600
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