Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler.
Posttranslational modifications play a key role in recruiting chromatin remodeling and modifying enzymes to specific regions of chromosomes to modulate chromatin structure. Alc1 (amplified in liver cancer 1), a member of the SNF2 ATPase superfamily with a carboxy-terminal macrodomain, is encoded by an oncogene implicated in the pathogenesis of hepatocellular ... carcinoma. Here we show that Alc1 interacts transiently with chromatin-associated proteins, including histones and the poly(ADP-ribose) polymerase Parp1. Alc1 ATPase and chromatin remodeling activities are strongly activated by Parp1 and its substrate NAD and require an intact macrodomain capable of binding poly(ADP-ribose). Alc1 is rapidly recruited to nucleosomes in vitro and to chromatin in cells when Parp1 catalyzes PAR synthesis. We propose that poly(ADP-ribosyl)ation of chromatin-associated Parp1 serves as a mechanism for targeting a SNF2 family remodeler to chromatin.
Mesh Terms:
Adenosine Triphosphatases, Adenosine Triphosphate, Carcinoma, Hepatocellular, Cell Line, Cell Line, Tumor, Chromatin, Chromatin Assembly and Disassembly, DNA Helicases, DNA-Binding Proteins, Hela Cells, Humans, Liver Neoplasms, Nucleosomes, Poly(ADP-ribose) Polymerases, Protein Processing, Post-Translational, Protein Structure, Tertiary, Transcription Factors
Adenosine Triphosphatases, Adenosine Triphosphate, Carcinoma, Hepatocellular, Cell Line, Cell Line, Tumor, Chromatin, Chromatin Assembly and Disassembly, DNA Helicases, DNA-Binding Proteins, Hela Cells, Humans, Liver Neoplasms, Nucleosomes, Poly(ADP-ribose) Polymerases, Protein Processing, Post-Translational, Protein Structure, Tertiary, Transcription Factors
Proc. Natl. Acad. Sci. U.S.A.
Date: Aug. 18, 2009
PubMed ID: 19666485
View in: Pubmed Google Scholar
Download Curated Data For This Publication
105225
Switch View:
- Interactions 15