Interaction of PRMT1 with BTG/TOB proteins in cell signalling: molecular analysis and functional aspects.

BACKGROUND: Several recent reports have connected protein methylation with differentiation. Furthermore, the BTG/TOB proteins have also been implicated in such control. BTG1 and 2 have been shown to interact with PRMT1 (predominant cellular arginine N-methyltransferase of type I). RESULTS: First, we have studied the interaction between PRMT1 and the proteins ...
of the BTG/TOB family. We show that boxC, a sequence present only in BTG1 and BTG2, is essential for this association. Using boxC peptide, we have investigated the importance of PRMT1/BTG protein association during type I protein methylation reactions. Finally, we show that the addition of boxC fused to penetratin interferes with the neuronal differentiation of PC12 cells and ES cell-derived neurones. CONCLUSIONS: Taken together, these results indicate that PRMT1/BTG proteins could play a key role in the arginine methylation-mediated signalling pathway as well as in neuronal differentiation.
Mesh Terms:
Animals, Apoptosis, Carrier Proteins, Cell Cycle, Cell Differentiation, DNA Methylation, Immediate-Early Proteins, Neoplasm Proteins, Neurons, PC12 Cells, Protein-Arginine N-Methyltransferases, Rats, Sequence Analysis, Protein, Signal Transduction, Stem Cells
Genes Cells
Date: Jan. 01, 2002
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