Rapid blue-light-mediated induction of protein interactions in living cells.
Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution. We demonstrate ... the utility of this system by inducing protein translocation, transcription and Cre recombinase-mediated DNA recombination using light.
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Date: Oct. 31, 2010
PubMed ID: 21037589
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