Epitope tag-induced synthetic lethality between cohesin subunits and Ctf7/Eco1 acetyltransferase.

Ctf7/Eco1-dependent acetylation of Smc3 is essential for sister chromatid cohesion. Here, we use epitope tag-induced lethality in cells diminished for Ctf7/Eco1 activity to map cohesin architecture in vivo. Tagging either Smc1 or Mcd1/Scc1, but not Scc3/Irr1, appears to abolish access to Smc3 in ctf7/eco1 mutant cells, suggesting that Smc1 and ...
Smc3 head domains are in direct contact with each other and also with Mcd1/Scc1. Thus, cohesin complexes may be much more compact than commonly portrayed. We further demonstrate that mutation in ELG1 or RFC5 anti-establishment genes suppress tag-induced lethality, consistent with the notion that the replication fork regulates Ctf7/Eco1.
Mesh Terms:
Acetylation, Acetyltransferases, Carrier Proteins, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, Nuclear Proteins, Protein Conformation, Protein Interaction Mapping, Protein Multimerization, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
FEBS Lett.
Date: Sep. 24, 2010
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