Functional selectivity of recombinant mammalian SWI/SNF subunits.
The SWI/SNF family of chromatin-remodeling complexes plays a key role in facilitating the binding of specific transcription factors to nucleosomal DNA in diverse organisms from yeast to man. Yet the process by which SWI/SNF and other chromatin-remodeling complexes activate specific subsets of genes is poorly understood. We show that mammalian ... SWI/SNF regulates transcription from chromatin-assembled genes in a factor-specific manner in vitro. The DNA-binding domains (DBDs) of several zinc finger proteins, including EKLF, interact directly with SWI/SNF to generate DNase I hypersensitivity within the chromatin-assembled beta-globin promoter. Interestingly, we find that two SWI/SNF subunits (BRG1 and BAF155) are necessary and sufficient for targeted chromatin remodeling and transcriptional activation by EKLF in vitro. Remodeling is achieved with only the BRG1-BAF155 minimal complex and the EKLF zinc finger DBD, whereas transcription requires, in addition, an activation domain. In contrast, the BRG1-BAF155 complex does not interact or function with two unrelated transcription factors, TFE3 and NF-kappaB. We conclude that specific domains of certain transcription factors differentially target SWI/SNF complexes to chromatin in a gene-selective manner and that individual SWI/SNF subunits play unique roles in transcription factor-directed nucleosome remodeling.
Mesh Terms:
Adenosine Triphosphatases, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Binding Sites, Chromatin, Chromosomal Proteins, Non-Histone, DNA Helicases, DNA-Binding Proteins, Erythroid-Specific DNA-Binding Factors, Globins, HIV-1, Kruppel-Like Transcription Factors, Models, Genetic, NF-kappa B, Nuclear Proteins, Nucleosomes, Promoter Regions, Genetic, Protein Binding, Protein Subunits, Recombinant Proteins, Transcription Factors, Transcriptional Activation, Zinc Fingers
Adenosine Triphosphatases, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Binding Sites, Chromatin, Chromosomal Proteins, Non-Histone, DNA Helicases, DNA-Binding Proteins, Erythroid-Specific DNA-Binding Factors, Globins, HIV-1, Kruppel-Like Transcription Factors, Models, Genetic, NF-kappa B, Nuclear Proteins, Nucleosomes, Promoter Regions, Genetic, Protein Binding, Protein Subunits, Recombinant Proteins, Transcription Factors, Transcriptional Activation, Zinc Fingers
Genes Dev.
Date: Oct. 01, 2000
PubMed ID: 11018012
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