ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
We have identified two highly conserved RING finger proteins, ROC1 and ROC2, that are homologous to APC11, a subunit of the anaphase-promoting complex. ROC1 and ROC2 commonly interact with all cullins while APC11 specifically interacts with APC2, a cullin-related APC subunit. YeastROC1 encodes an essential gene whose reduced expression resulted ... in multiple, elongated buds and accumulation of Sic1p and Cln2p. ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1- and E2-dependent manner. ROC1 mutations completely abolished their ligase activity without noticeable changes in associated proteins. Ubiquitination of phosphorylated I kappa B alpha can be catalyzed by the ROC1 immunocomplex in vitro. Hence, combinations of ROC/APC11 and cullin proteins proteins potentially constitute a wide variety of ubiquitin ligases.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Cell Cycle Proteins, Cell Line, Cullin Proteins, F-Box Proteins, Fungal Proteins, Humans, Ligases, Molecular Sequence Data, Peptide Synthases, Phenotype, SKP Cullin F-Box Protein Ligases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases, Ubiquitins
Amino Acid Sequence, Base Sequence, Cell Cycle Proteins, Cell Line, Cullin Proteins, F-Box Proteins, Fungal Proteins, Humans, Ligases, Molecular Sequence Data, Peptide Synthases, Phenotype, SKP Cullin F-Box Protein Ligases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases, Ubiquitins
Mol. Cell
Date: Apr. 01, 1999
PubMed ID: 10230407
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