Involvement of CRAF1, a relative of TRAF, in CD40 signaling.
CD40 is a receptor on the surface of B lymphocytes, the activation of which leads to B cell survival, growth, and differentiation. A yeast two-hybrid screen identified a gene, CRAF1, encoding a protein that interacts directly with the CD40 cytoplasmic tail through a region of similarity to the tumor necrosis ... factor-alpha (TNF-alpha) receptor-associated factors. Overexpression of a truncated CRAF1 gene inhibited CD40-mediated up-regulation of CD23. A region of CRAF1 was similar to the TNF-alpha receptor-associated factors TRAF1 and TRAF2 and so defined a shared TRAF-C domain that was necessary and sufficient for CD40 binding and homodimerization. The CRAF1 sequence also predicted a long amphipathic helix, a pattern of five zinc fingers, and a zinc ring finger. It is likely that other members of the TNF receptor superfamily use CRAF-related proteins in their signal transduction processes.
Mesh Terms:
Amino Acid Sequence, Animals, Antigens, CD, Antigens, CD40, Antigens, Differentiation, B-Lymphocyte, Humans, Mice, Molecular Sequence Data, Protein Structure, Secondary, Proteins, Receptors, IgE, Sequence Homology, Amino Acid, Signal Transduction, TNF Receptor-Associated Factor 1, TNF Receptor-Associated Factor 2, TNF Receptor-Associated Factor 3, Up-Regulation, Zinc Fingers
Amino Acid Sequence, Animals, Antigens, CD, Antigens, CD40, Antigens, Differentiation, B-Lymphocyte, Humans, Mice, Molecular Sequence Data, Protein Structure, Secondary, Proteins, Receptors, IgE, Sequence Homology, Amino Acid, Signal Transduction, TNF Receptor-Associated Factor 1, TNF Receptor-Associated Factor 2, TNF Receptor-Associated Factor 3, Up-Regulation, Zinc Fingers
Science
Date: Mar. 10, 1995
PubMed ID: 7533327
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