C. elegans CED-12 acts in the conserved crkII/DOCK180/Rac pathway to control cell migration and cell corpse engulfment.
We have identified and characterized a novel C. elegans gene, ced-12, that functions in the conserved GTPase signaling pathway mediated by CED-2/Crkll, CED-5/DOCK180, and CED-10/Rac to control cell migration and phagocytosis of apoptotic cells. We provide evidence that ced-12 likely acts upstream of ced-10 during cell migration and phagocytosis and ... that CED-12 physically interacts with CED-5 and forms a ternary complex with CED-2 in vitro. We propose that the formation and localization of a CED-2-CED-5-CED-12 ternary complex to the plasma membrane activates CED-10, leading to the cytoskeletal reorganization that occurs in the polarized extension of cell surfaces in engulfing cells and migrating cells. We suggest that CED-12 counterparts in higher organisms regulate cytoskeleton dynamics, as CED-12 does in C. elegans.
Mesh Terms:
Amino Acid Sequence, Animals, Animals, Genetically Modified, Apoptosis, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Carrier Proteins, Cell Movement, Cloning, Molecular, Conserved Sequence, Cytoskeletal Proteins, Helminth Proteins, Membrane Proteins, Molecular Sequence Data, Mutation, Phagocytosis, Protein Kinases, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-crk, rac GTP-Binding Proteins
Amino Acid Sequence, Animals, Animals, Genetically Modified, Apoptosis, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Carrier Proteins, Cell Movement, Cloning, Molecular, Conserved Sequence, Cytoskeletal Proteins, Helminth Proteins, Membrane Proteins, Molecular Sequence Data, Mutation, Phagocytosis, Protein Kinases, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-crk, rac GTP-Binding Proteins
Dev. Cell
Date: Oct. 01, 2001
PubMed ID: 11703940
View in: Pubmed Google Scholar
Download Curated Data For This Publication
107374
Switch View:
- Interactions 4