Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.
Sentrin-1/SUMO-1 is a novel ubiquitin-like protein, which can covalently modify a limited number of cellular proteins. Here we report the identification of the sentrin-activating enzyme complex, which consists of two proteins AOS1 and UBA2. Human AOS1 is homologous to the N-terminal half of E1, whereas human UBA2 is homologous to ... the C-terminal half of E1. The human UBA2 gene is located on chromosome 19q12. Human UBA2 could form a beta-mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8, in the presence of AOS1. Identification of human UBA2 and AOS1 should allow a more detailed analysis of the enzymology of the activation of ubiquitin-like proteins.
Mesh Terms:
Amino Acid Sequence, Chromosome Mapping, Chromosomes, Cloning, Molecular, Enzyme Activation, Humans, Ligases, Molecular Sequence Data, SUMO-1 Protein, Sequence Homology, Amino Acid, Ubiquitin-Activating Enzymes, Ubiquitin-Protein Ligases, Ubiquitins
Amino Acid Sequence, Chromosome Mapping, Chromosomes, Cloning, Molecular, Enzyme Activation, Humans, Ligases, Molecular Sequence Data, SUMO-1 Protein, Sequence Homology, Amino Acid, Ubiquitin-Activating Enzymes, Ubiquitin-Protein Ligases, Ubiquitins
FEBS Lett.
Date: Apr. 01, 1999
PubMed ID: 10217437
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