A novel protein phosphatase 2C family member (PP2Czeta) is able to associate with ubiquitin conjugating enzyme 9.
In this study we have cloned a novel member of mouse protein phosphatase 2C family, PP2Czeta, which is composed of 507 amino acids and has a unique N-terminal region. The overall similarity of the amino acid sequence between PP2Czeta and PP2Calpha was 22%. On Northern blot analysis PP2Czeta was found ... to be expressed specifically in the testicular germ cells. PP2Czeta expressed in COS7 cells was able to associate with ubiquitin conjugating enzyme 9 (UBC9) and the association was enhanced by co-expression of small ubiquitin-related modifier-1 (SUMO-1), suggesting that PP2Czeta exhibits its specific role through its SUMO-induced recruitment to UBC9.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, COS Cells, DNA, Complementary, Escherichia coli, Isoenzymes, Ligases, Molecular Sequence Data, Phosphoprotein Phosphatases, Protein Binding, Recombinant Proteins, Sequence Homology, Amino Acid, Ubiquitin-Conjugating Enzymes
Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, COS Cells, DNA, Complementary, Escherichia coli, Isoenzymes, Ligases, Molecular Sequence Data, Phosphoprotein Phosphatases, Protein Binding, Recombinant Proteins, Sequence Homology, Amino Acid, Ubiquitin-Conjugating Enzymes
FEBS Lett.
Date: Mar. 13, 2003
PubMed ID: 12633878
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