The novel protein complex with SMARCAD1/KIAA1122 binds to the vicinity of TSS.
The SMARCAD1/KIAA1122 protein is structurally classified into the SWI2/SNF2 superfamily of DNA-dependent ATPases that are catalytic subunits of chromatin-remodeling complexes. Although the importance of other members of the SWR1-like subfamily in chromatin remodeling (EP400, INOC1, and SRCAP) has already been elucidated, the biological function of SMARCAD1/KIAA1122 in transcriptional regulation remains ... to be clarified. To gain insight into the role of this protein, we generated a specific antibody against SMARCAD1/KIAA1122 and used it for chromatin and protein immunoprecipitation assays. We employed high-resolution genome tiling microarrays in chromatin immunoprecipitation and found the binding sites of SMARCAD1/KIAA1122 in the vicinity of the transcriptional start site of 69 candidate target genes. In the protein immunoprecipitation assay, we found that endogenous SMARCAD1/KIAA1122 binds with TRIM28, a recently highlighted transcriptional regulator in the cancer field. From these findings, we propose a novel model for gene regulation via the SMARCAD1/KIAA1122 protein complex.
Mesh Terms:
Amino Acid Sequence, Animals, Gene Expression Profiling, Gene Expression Regulation, Humans, Leukemia, Lymphoid, Mice, Molecular Sequence Data, Multiprotein Complexes, Neoplasms, Germ Cell and Embryonal, Nuclear Proteins, Oligonucleotide Array Sequence Analysis, Phylogeny, Protein Binding, Transcription Initiation Site
Amino Acid Sequence, Animals, Gene Expression Profiling, Gene Expression Regulation, Humans, Leukemia, Lymphoid, Mice, Molecular Sequence Data, Multiprotein Complexes, Neoplasms, Germ Cell and Embryonal, Nuclear Proteins, Oligonucleotide Array Sequence Analysis, Phylogeny, Protein Binding, Transcription Initiation Site
J. Mol. Biol.
Date: Oct. 03, 2008
PubMed ID: 18675275
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