A DBF4 mutant that contributes to bypass the RAD53 mediated block of origins of replication in response to genotoxic stress.
An intra-S phase checkpoint slows the rate of DNA replication in response to DNA damage and replication fork blocks in eukaryotic cells. In the budding yeast Saccharomyces cerevisiae such down-regulation is achieved through the Rad53 kinase dependent block of origins of replication. We have identified the Rad53 phosphorylation sites on ... Dbf4, the activator subunit of the essential S-phase kinase DDK (Dbf4 Dependent Kinase), and generated an unphosphorylatable Dbf4 mutant (dbf4(7A)). We show here that dbf4(7A) is a bona fide intra-S phase checkpoint bypass allele, that contributes to abrogate the Rad53 block of origin firing in response to genotoxic stress.
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Date: Nov. 23, 2010
PubMed ID: 21098477
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