Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter.
Activation of the human immunodeficiency virus type-1 (HIV-1) promoter in infected cells requires the sequential recruitment of several cellular factors to facilitate the formation of a processive elongation complex. The nucleosomal reorganization of the HIV-1 long terminal repeat (LTR) observed upon Tat stimulation suggests that chromatin-remodeling complexes could play a ... role during this process. Here, we reported that Tat interacts directly with Brm, a DNA-dependent ATPase subunit of the SWI/SNF chromatin-remodeling complex, to activate the HIV-1 LTR. Inhibition of Brm via small interfering RNAs impaired Tat-mediated transactivation of an integrated HIV-1 promoter. Furthermore, Brm is recruited in vivo to the HIV-1 LTR in a Tat-dependent manner. Interestingly, we found that Tat/Brm interaction is regulated by Tat lysine 50 acetylation. These data show the requirement of Tat-mediated recruitment of SWI/SNF chromatin-remodeling complex to HIV-1 promoter in the activation of the LTR.
Mesh Terms:
Acetylation, Amino Acid Motifs, Arginine, Cell Line, Gene Products, tat, HIV-1, Humans, Lysine, Mutation, Promoter Regions, Genetic, Protein Binding, Protein Subunits, Terminal Repeat Sequences, Transcription Factors, tat Gene Products, Human Immunodeficiency Virus
Acetylation, Amino Acid Motifs, Arginine, Cell Line, Gene Products, tat, HIV-1, Humans, Lysine, Mutation, Promoter Regions, Genetic, Protein Binding, Protein Subunits, Terminal Repeat Sequences, Transcription Factors, tat Gene Products, Human Immunodeficiency Virus
EMBO J.
Date: Apr. 19, 2006
PubMed ID: 16601680
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