An IAP-IAP complex inhibits apoptosis.
Regulators of apoptosis are thought to work in concert, but the molecular interactions of this process are not understood. Here, we show that in response to cell death stimulation, survivin, a member of the inhibitor of apoptosis (IAP) gene family, associates with another IAP protein, XIAP, via conserved baculovirus IAP ... repeats. Formation of a survivin-XIAP complex promotes increased XIAP stability against ubiquitination/proteasomal destruction and synergistic inhibition of apoptosis, which is abolished in XIAP(-/-) cells. Therefore, orchestration of an IAP-IAP complex regulates apoptosis.
Mesh Terms:
Animals, Apoptosis, Baculoviridae, Caspase 9, Caspases, Cell Death, Cell Line, Cell Line, Tumor, Cysteine Endopeptidases, Dose-Response Relationship, Drug, Fibroblasts, Gene Expression Regulation, Glutathione Transferase, Humans, Immunoblotting, Mice, Mice, Transgenic, Microtubule-Associated Proteins, Multienzyme Complexes, Neoplasm Proteins, Precipitin Tests, Proteasome Endopeptidase Complex, Protein Binding, Proteins, Recombinant Proteins, Time Factors, Transfection, Transgenes, Ubiquitin, X-Linked Inhibitor of Apoptosis Protein
Animals, Apoptosis, Baculoviridae, Caspase 9, Caspases, Cell Death, Cell Line, Cell Line, Tumor, Cysteine Endopeptidases, Dose-Response Relationship, Drug, Fibroblasts, Gene Expression Regulation, Glutathione Transferase, Humans, Immunoblotting, Mice, Mice, Transgenic, Microtubule-Associated Proteins, Multienzyme Complexes, Neoplasm Proteins, Precipitin Tests, Proteasome Endopeptidase Complex, Protein Binding, Proteins, Recombinant Proteins, Time Factors, Transfection, Transgenes, Ubiquitin, X-Linked Inhibitor of Apoptosis Protein
J. Biol. Chem.
Date: Aug. 13, 2004
PubMed ID: 15218035
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