Selective potentiation of Stat-dependent gene expression by collaborator of Stat6 (CoaSt6), a transcriptional cofactor.

The molecular mechanisms by which transcription is selectively activated and precisely controlled by signal transducer and activator of transcription (Stat) factors represent a central issue in cytokine-mediated cellular responses. Stat6 mediates responses to IL-4 and antagonizes Stat1 activated by IFN-gamma. We have discovered that Stat6 binds to collaborator of Stat6 ...
(CoaSt6), a protein that lacks conventional coactivator motifs but contains three iterations of a domain found in the variant histone macroH2A. Although macroH2A participates in transcriptional silencing, the macro domains of CoaSt6 increased IL-4-induced gene expression. Moreover, CoaSt6 amplified Stat6-mediated but not IFN-gamma-induced gene expression, providing evidence of a selective coregulator of Stat-mediated gene transcription.
Mesh Terms:
Animals, Base Sequence, Cloning, Molecular, DNA, Gene Expression, Histones, Humans, Interleukin-4, Mice, Molecular Sequence Data, Mutagenesis, Poly Adenosine Diphosphate Ribose, Poly(ADP-ribose) Polymerases, Protein Structure, Tertiary, Recombinant Proteins, STAT6 Transcription Factor, Trans-Activators, Transfection, Two-Hybrid System Techniques
Proc. Natl. Acad. Sci. U.S.A.
Date: Mar. 14, 2006
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