The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription.
Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein-protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and ... SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.
Mesh Terms:
Adenosine Triphosphatases, Cell Nucleus, Chromatin Assembly and Disassembly, Chromosomal Proteins, Non-Histone, Hela Cells, Humans, Intracellular Signaling Peptides and Proteins, Myosin Type I, Nuclear Proteins, Protein Binding, RNA Polymerase I, Transcription Factors, Transcription, Genetic
Adenosine Triphosphatases, Cell Nucleus, Chromatin Assembly and Disassembly, Chromosomal Proteins, Non-Histone, Hela Cells, Humans, Intracellular Signaling Peptides and Proteins, Myosin Type I, Nuclear Proteins, Protein Binding, RNA Polymerase I, Transcription Factors, Transcription, Genetic
EMBO Rep.
Date: May. 01, 2006
PubMed ID: 16514417
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