Two histone marks establish the inner centromere and chromosome bi-orientation.
For proper partitioning of chromosomes in mitosis, the chromosomal passenger complex (CPC) including Aurora B and survivin must be localized at the center of paired kinetochores, at the site called the inner centromere. It is largely unknown what defines the inner centromere and how the CPC is targeted to this ... site. Here, we show that the phosphorylation of histone H3-threonine 3 (H3-pT3) mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 (H2A-S121) phosphorylation in targeting the CPC to the inner centromere in fission yeast and human cells. H3-pT3 promotes nucleosome binding of survivin, whereas phosphorylated H2A-S121 facilitates the binding of shugoshin, the centromeric CPC adaptor. Haspin colocalizes with cohesin by associating with Pds5, whereas Bub1 localizes at kinetochores. Thus, the inner centromere is defined by intersection of two histone kinases.
Mesh Terms:
Amino Acid Sequence, Cell Cycle Proteins, Centromere, Chromatin, Chromosomal Proteins, Non-Histone, Chromosome Segregation, Chromosomes, Fungal, Chromosomes, Human, Hela Cells, Heterochromatin, Histones, Humans, Intracellular Signaling Peptides and Proteins, Kinetochores, Microtubule-Associated Proteins, Mitosis, Molecular Sequence Data, Mutation, Nucleosomes, Phosphorylation, Protein Binding, Protein Interaction Domains and Motifs, Protein-Serine-Threonine Kinases, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Serine, Threonine
Amino Acid Sequence, Cell Cycle Proteins, Centromere, Chromatin, Chromosomal Proteins, Non-Histone, Chromosome Segregation, Chromosomes, Fungal, Chromosomes, Human, Hela Cells, Heterochromatin, Histones, Humans, Intracellular Signaling Peptides and Proteins, Kinetochores, Microtubule-Associated Proteins, Mitosis, Molecular Sequence Data, Mutation, Nucleosomes, Phosphorylation, Protein Binding, Protein Interaction Domains and Motifs, Protein-Serine-Threonine Kinases, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Serine, Threonine
Science
Date: Oct. 08, 2010
PubMed ID: 20929775
View in: Pubmed Google Scholar
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