EphA receptors regulate growth cone dynamics through the novel guanine nucleotide exchange factor ephexin.
Eph receptors transduce short-range repulsive signals for axon guidance by modulating actin dynamics within growth cones. We report the cloning and characterization of ephexin, a novel Eph receptor-interacting protein that is a member of the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho GTPases. Ephrin-A stimulation of EphA ... receptors modulates the activity of ephexin leading to RhoA activation, Cdc42 and Rac1 inhibition, and cell morphology changes. In addition, expression of a mutant form of ephexin in primary neurons interferes with ephrin-A-induced growth cone collapse. The association of ephexin with Eph receptors constitutes a molecular link between Eph receptors and the actin cytoskeleton and provides a novel mechanism for achieving highly localized regulation of growth cone motility.
Mesh Terms:
Actins, Amino Acid Sequence, Animals, Brain Chemistry, Cells, Cultured, Cloning, Molecular, Embryo, Mammalian, Ephrin-A1, Eye, Fetal Proteins, Growth Cones, Guanine Nucleotide Exchange Factors, Immunoblotting, In Situ Hybridization, Mice, Molecular Sequence Data, Nerve Tissue Proteins, Phylogeny, Protein Structure, Tertiary, Proteins, Rats, Receptor Protein-Tyrosine Kinases, Signal Transduction, Two-Hybrid System Techniques, rho GTP-Binding Proteins
Actins, Amino Acid Sequence, Animals, Brain Chemistry, Cells, Cultured, Cloning, Molecular, Embryo, Mammalian, Ephrin-A1, Eye, Fetal Proteins, Growth Cones, Guanine Nucleotide Exchange Factors, Immunoblotting, In Situ Hybridization, Mice, Molecular Sequence Data, Nerve Tissue Proteins, Phylogeny, Protein Structure, Tertiary, Proteins, Rats, Receptor Protein-Tyrosine Kinases, Signal Transduction, Two-Hybrid System Techniques, rho GTP-Binding Proteins
Cell
Date: Apr. 20, 2001
PubMed ID: 11336673
View in: Pubmed Google Scholar
Download Curated Data For This Publication
11001
Switch View:
- Interactions 1