53BP1, a mediator of the DNA damage checkpoint.
53BP1 binds to the tumor suppressor protein p53 and has a potential role in DNA damage responses. We used small interfering RNA (siRNA) directed against 53BP1 in mammalian cells to demonstrate that 53BP1 is a key transducer of the DNA damage checkpoint signal. 53BP1 was required for p53 accumulation, G2-M ... checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. 53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.
Mesh Terms:
BRCA1 Protein, Carrier Proteins, Cell Cycle Proteins, DNA, DNA Damage, G2 Phase, Histones, Humans, Intracellular Signaling Peptides and Proteins, Mitosis, Nuclear Proteins, Phosphoproteins, Phosphorylation, Protein Kinases, Protein-Serine-Threonine Kinases, RNA, Small Interfering, Radiation, Ionizing, S Phase, Signal Transduction, Tumor Suppressor Protein p53
BRCA1 Protein, Carrier Proteins, Cell Cycle Proteins, DNA, DNA Damage, G2 Phase, Histones, Humans, Intracellular Signaling Peptides and Proteins, Mitosis, Nuclear Proteins, Phosphoproteins, Phosphorylation, Protein Kinases, Protein-Serine-Threonine Kinases, RNA, Small Interfering, Radiation, Ionizing, S Phase, Signal Transduction, Tumor Suppressor Protein p53
Science
Date: Nov. 15, 2002
PubMed ID: 12364621
View in: Pubmed Google Scholar
Download Curated Data For This Publication
110554
Switch View:
- Interactions 1