NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP, a PEST-containing nuclear protein.
We previously reported the association of a novel Np95/ICBP90-like RING finger protein (NIRF) with a novel PEST-containing nuclear protein (PCNP). NIRF is a nuclear protein with a ubiquitin-like domain, a PHD finger, a YDG/SRA domain, Rb-binding motifs and a RING finger. In this study, we showed that NIRF has auto-ubiquitination ... activity, the hallmark of a ubiquitin ligase. PCNP was readily ubiquitinated in 293 and COS-7 cells, and NIRF ubiquitinated PCNP in vitro as well as in vivo. Considering that NIRF is implicated in cell cycle regulation, these findings suggest that NIRF and PCNP are a ubiquitin ligase and its substrate, respectively, and may constitute a novel signaling pathway with some relation to cell proliferation.
Mesh Terms:
Animals, COS Cells, Catalysis, Cell Line, Cercopithecus aethiops, Humans, Kinetics, Molecular Sequence Data, Nuclear Proteins, Rabbits, Transfection, Ubiquitin, Ubiquitin-Protein Ligases
Animals, COS Cells, Catalysis, Cell Line, Cercopithecus aethiops, Humans, Kinetics, Molecular Sequence Data, Nuclear Proteins, Rabbits, Transfection, Ubiquitin, Ubiquitin-Protein Ligases
FEBS Lett.
Date: Jan. 16, 2004
PubMed ID: 14741369
View in: Pubmed Google Scholar
Download Curated Data For This Publication
110895
Switch View:
- Interactions 5
- PTM Genes 2