Critical roles of TRAF2 and TRAF5 in tumor necrosis factor-induced NF-kappa B activation and protection from cell death.
Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) were identified as signal transducers for the TNF receptor superfamily. However, the exact roles of TRAF2 and TRAF5 in TNF-induced NF-kappaB activation still remain controversial. To address this issue, we generated TRAF2 and TRAF5 double knockout (DKO) mice. TNF- but not interleukin-1-induced nuclear ... translocation of NF-kappaB was severely impaired in murine embryonic fibroblasts (MEFs) derived from DKO mice. Moreover, DKO MEFs were more susceptible to TNF-induced cytotoxicity than TRAF2 knockout MEFs. Collectively, these results indicate that both TRAF2 and TRAF5 are involved in TNF-induced NF-kappaB activation and protection from cell death.
Mesh Terms:
Animals, Blotting, Western, Cell Death, Dose-Response Relationship, Drug, Enzyme Activation, Fibroblasts, Humans, Interleukin-1, Mice, Mice, Knockout, NF-kappa B, Plasmids, Precipitin Tests, Protein Binding, Proteins, Recombinant Proteins, Signal Transduction, TNF Receptor-Associated Factor 2, TNF Receptor-Associated Factor 5, Tumor Necrosis Factor-alpha
Animals, Blotting, Western, Cell Death, Dose-Response Relationship, Drug, Enzyme Activation, Fibroblasts, Humans, Interleukin-1, Mice, Mice, Knockout, NF-kappa B, Plasmids, Precipitin Tests, Protein Binding, Proteins, Recombinant Proteins, Signal Transduction, TNF Receptor-Associated Factor 2, TNF Receptor-Associated Factor 5, Tumor Necrosis Factor-alpha
J. Biol. Chem.
Date: Sep. 28, 2001
PubMed ID: 11479302
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