BAF complex is closely related to and interacts with NF1/CTF and RNA polymerase II in gene transcriptional activation.
Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subunit of Brg-associated factor complexes. In the present study, we investigated the spatial relationship between Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase II (RNAP ... II) upon gene transcriptional activation in vivo by employing immuno-gold labeling. The data showed that Brg1 was closely co-localized with NF1/CTF and RNAP II in HeLa cells. Moreover, the co-immunoprecipitation assay further revealed that Brg1 can be isolated together with NF1/CTF and RNAP II in the ConA-stimulated, but not the resting, T lymphocyte. The combined results suggested that BAF complexes can interact with NF1/CTF and RNAP II, and this interaction is closely dependent on the activation of gene transcription.
Mesh Terms:
Animals, CCAAT-Enhancer-Binding Proteins, DNA Helicases, Hela Cells, Humans, Immunohistochemistry, Jurkat Cells, Mice, NFI Transcription Factors, Nuclear Proteins, RNA Polymerase II, T-Lymphocytes, Transcription Factors, Transcriptional Activation
Animals, CCAAT-Enhancer-Binding Proteins, DNA Helicases, Hela Cells, Humans, Immunohistochemistry, Jurkat Cells, Mice, NFI Transcription Factors, Nuclear Proteins, RNA Polymerase II, T-Lymphocytes, Transcription Factors, Transcriptional Activation
Acta Biochim. Biophys. Sin. (Shanghai)
Date: Jul. 01, 2005
PubMed ID: 15999204
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