AMY-1, a c-Myc-binding protein, is localized in the mitochondria of sperm by association with S-AKAP84, an anchor protein of cAMP-dependent protein kinase.

We have reported that a novel c-Myc-binding protein, AMY-1 (associate of Myc-1), stimulated the transcription activity of c-Myc. To access the molecular function of AMY-1, a two-hybrid screening of cDNAs encoding AMY-1-binding proteins was carried out with AMY-1 as a bait using a human HeLa cDNA library, and a clone ...
encoding cAMP-dependent protein kinase anchor protein 149 (AKAP149), was obtained. AMY-1 was found to bind in vitro and in vivo to the regulatory subunit II binding region of AKAP149 and S-AKAP84, a splicing variant of AKAP149 expressed in the testis. AMY-1 was expressed postmeiotically in the testis, as S-AKAP84 was expressed. Furthermore, S-AKAP84 and regulatory subunit II, a regulatory subunit of cAMP-dependent protein kinase, made a ternary complex in cells, and AMY-1 was localized in the mitochondria of HeLa and sperm in association with AKAP149 and S-AKAP84, respectively. These results suggest that AMY-1 plays a role in spermatogenesis.
Mesh Terms:
A Kinase Anchor Proteins, Adaptor Proteins, Signal Transducing, Alternative Splicing, Animals, Blotting, Northern, Carrier Proteins, Cell Line, Cyclic AMP-Dependent Protein Kinases, DNA-Binding Proteins, Fluorescent Antibody Technique, Indirect, Hela Cells, Humans, Male, Membrane Proteins, Mice, Mitochondria, Plasmids, Protein Binding, Protein Transport, Proto-Oncogene Proteins c-myc, Reverse Transcriptase Polymerase Chain Reaction, Salivary alpha-Amylases, Spermatogenesis, Spermatozoa, Testis, Tissue Distribution, Transcription Factors, Two-Hybrid System Techniques
J. Biol. Chem.
Date: Sep. 28, 2001
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