Aberrantly expressed recoverin is functionally associated with G-protein-coupled receptor kinases in cancer cell lines.

Cancer-associated retinopathy (CAR) is an ocular manifestation of a paraneoplastic syndrome whereby immunological reactions toward recoverin (Rec), a retina-specific Ca(2+) binding protein, and its aberrant expression in tumor cells lead to the retinal degeneration. To elucidate functional roles of the aberrantly expression in cancer cells, we performed immunoprecipitation using anti-human ...
Rec mAb. We observed co-precipitation of G-protein-coupled receptor kinases (GRKs) and caveolin-1 with Rec from cell lysates of 293 or SSTW cells. Immunocytochemistry revealed that immunoreactivities toward Rec within the cancer cells were almost identical to those toward GRKs and caveolin-1. The present data strongly suggest that aberrantly expressed Rec should be involved in the GRK-dependent cellular regulation in cancer cells.
Mesh Terms:
Antibodies, Monoclonal, Calcium-Binding Proteins, Caveolin 1, Caveolins, Cell Line, Cyclic AMP-Dependent Protein Kinases, Eye Proteins, G-Protein-Coupled Receptor Kinase 2, G-Protein-Coupled Receptor Kinase 5, G-Protein-Coupled Receptor Kinases, GTP-Binding Proteins, Hippocalcin, Humans, Immunohistochemistry, Kidney, Lipoproteins, Nerve Tissue Proteins, Precipitin Tests, Protein Binding, Protein-Serine-Threonine Kinases, Recoverin, Stomach Neoplasms, Tumor Cells, Cultured, beta-Adrenergic Receptor Kinases
Biochem. Biophys. Res. Commun.
Date: Jan. 17, 2003
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