KAP1 regulates type I interferon/STAT1-mediated IRF-1 gene expression.
Signal transducers and activators of transcription (STATs) mediate cell proliferation, differentiation, and survival in immune responses, hematopoiesis, neurogenesis, and other biological processes. Recently, we showed that KAP1 is a novel STAT-binding partner that regulates STAT3-mediated transactivation. KAP1 is a universal co-repressor protein for the KRAB zinc finger protein superfamily of ... transcriptional repressors. In this study, we found KAP1-dependent repression of interferon (IFN)/STAT1-mediated signaling. We also demonstrated that endogenous KAP1 associates with endogenous STAT1 in vivo. Importantly, a small-interfering RNA-mediated reduction in KAP1 expression enhanced IFN-induced STAT1-dependent IRF-1 gene expression. These results indicate that KAP1 may act as an endogenous regulator of the IFN/STAT1 signaling pathway.
Mesh Terms:
DNA-Binding Proteins, Gene Expression, Hela Cells, Histone Deacetylases, Humans, Immunoprecipitation, Interferon Regulatory Factor-1, Interferon Type I, RNA, Small Interfering, Repressor Proteins, STAT1 Transcription Factor, Transcriptional Activation
DNA-Binding Proteins, Gene Expression, Hela Cells, Histone Deacetylases, Humans, Immunoprecipitation, Interferon Regulatory Factor-1, Interferon Type I, RNA, Small Interfering, Repressor Proteins, STAT1 Transcription Factor, Transcriptional Activation
Biochem. Biophys. Res. Commun.
Date: May. 30, 2008
PubMed ID: 18381204
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