The Drosophila methyl-DNA binding protein MBD2/3 interacts with the NuRD complex via p55 and MI-2.
Methyl-DNA binding proteins help to translate epigenetic information encoded by DNA methylation into covalent histone modifications. MBD2/3 is the only candidate gene in the Drosophila genome with extended homologies to mammalian MBD2 and MBD3 proteins, which represent a co-repressor and an integral component of the Nucleosome Remodelling and Deacetylase (NuRD) ... complex, respectively. An association of Drosophila MBD2/3 with the Drosophila NuRD complex has been suggested previously. We have now analyzed the molecular interactions between MBD2/3 and the NuRD complex in greater detail.The two MBD2/3 isoforms precisely cofractionated with NuRD proteins during gel filtration of extracts derived from early and late embryos. In addition, we demonstrate that MBD2/3 forms multimers, and engages in specific interactions with the p55 and MI-2 subunits of the Drosophila NuRD complex.Our data provide novel insights into the association between Drosophila MBD2/3 and NuRD proteins. Additionally, this work provides a first analysis of the architecture of the Drosophila NuRD complex.
Mesh Terms:
Adenosine Triphosphatases, Animals, Autoantigens, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Histone Deacetylases, Mi-2 Nucleosome Remodeling and Deacetylase Complex, Molecular Chaperones, Protein Isoforms, Retinoblastoma-Binding Protein 4, Two-Hybrid System Techniques
Adenosine Triphosphatases, Animals, Autoantigens, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Histone Deacetylases, Mi-2 Nucleosome Remodeling and Deacetylase Complex, Molecular Chaperones, Protein Isoforms, Retinoblastoma-Binding Protein 4, Two-Hybrid System Techniques
BMC Mol. Biol.
Date: Oct. 29, 2004
PubMed ID: 15516265
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