The brain-specific actin-related protein ArpN alpha interacts with the transcriptional co-repressor CtBP.

Actin-related protein (Arp) is found in many chromatin remodeling and histone acetyltransferase complexes. We previously identified ArpN alpha as an isoform of ArpN beta/BAF53, which is included in mammalian SWI/SNF chromatin remodeling complex, and showed that ArpN alpha is a potential component of the complex. Although it has a structure ...
highly similar to ArpN beta/BAF53, ArpN alpha is expressed exclusively in brain and in neural differentiated embryonal carcinoma cells. Since ArpN alpha possesses a region that shows low similarity to ArpN beta/BAF53, we hypothesized that proteins interacting with this region contribute to the ArpN alpha-specific function in brain. Here we showed that ArpN alpha, but not ArpN beta/BAF53, interacts with the transcriptional co-repressor CtBP (C-terminal binding protein). Transactivation by the SWI/SNF complex and glucocorticoid receptor was repressed by the CtBP in the presence of ArpN alpha. These findings suggest that SWI/SNF complex containing ArpN alpha might regulate certain genes involved in brain development and/or its function differently from SWI/SNF complex containing ArpN beta/BAF53.
Mesh Terms:
Actins, Alcohol Oxidoreductases, Amino Acid Sequence, Animals, Brain, COS Cells, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Gene Expression Regulation, Genes, Reporter, Humans, Mice, Models, Molecular, Molecular Sequence Data, Nuclear Proteins, Phosphoproteins, Promoter Regions, Genetic, Protein Isoforms, Protein Structure, Tertiary, Repressor Proteins, Sequence Alignment, Transcription Factors, Transcriptional Activation, Two-Hybrid System Techniques
Biochem. Biophys. Res. Commun.
Date: Feb. 07, 2003
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