Cloning and characterization of BAI-associated protein 1: a PDZ domain-containing protein that interacts with BAI1.

Brain-specific angiogenesis inhibitor 1 (BAI1), which is a p53-target gene specifically expressed in brain, encodes a seven-span transmembrane protein. Using a two-hybrid system, we isolated a cDNA that encodes a protein, named BAP1 (BAI1-associated protein), which interacts with the cytoplasmic region of BAI1. BAP1 is a novel member of the ...
MAGUK (membrane-associated guanylate kinase homologue) family; it possesses a guanylate kinase domain, WW domains, and multiple PDZ domains. Interaction between BAI1 and BAP1 was mediated by a QTEV motif in the carboxy-terminal region of BAI1 and PDZ domains of BAP1. By immunocytochemical analysis of COS-7 cells transfected with BAI1 and BAP1, both products were co-localized at the cytoplasmic membrane, especially at cell-cell junctions. Cells transfected with BAI1 formed filopodia-like cytoplasmic extensions. These results suggest that BAI1 and BAP1 might be involved in cell adhesion and signal transduction in brain.
Mesh Terms:
Amino Acid Sequence, Animals, Brain, COS Cells, Cell Adhesion, Cell Adhesion Molecules, Neuronal, Chromosome Mapping, Chromosomes, Human, Pair 3, Cloning, Molecular, Drosophila Proteins, Guanylate Kinase, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Insect Proteins, Molecular Sequence Data, Neovascularization, Physiologic, Nerve Tissue Proteins, Nucleoside-Phosphate Kinase, RNA, Messenger, Recombinant Fusion Proteins, Sequence Homology, Amino Acid, Signal Transduction, Transfection
Biochem. Biophys. Res. Commun.
Date: Jun. 29, 1998
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