Rfc5p regulates alternate RFC complex functions in sister chromatid pairing reactions in budding yeast.

Sister chromatid pairing reactions, termed cohesion establishment, occur during S-phase and appear to be regulated by Replication Factor C (RFC) complexes. For instance, RFCs that contain Ctf18p exhibit pro-establishment activities while those that contain Elg1p exhibit anti-establishment activities. It remains unknown whether Ctf18p-RFC and Elg1p-RFC functions are simply opposing or ...
instead reveal complicated and non-parallel regulatory mechanisms. To better understand the nature of these novel pathways, we analyzed the small RFC subunit Rfc5p that is common to both Ctf18p-RFC and Elg1p-RFC. Despite this commonality, the data show that diminished Rfc5p function rescues ctf7/eco1 mutant cell phenotypes, revealing that Rfc5p promotes anti-establishment activities. This rescue is specific to establishment pathways in that rfc5-1 greatly accentuates growth defects when expressed in scc2 (deposition), mcd1/scc1 or smc3 (cohesion maintenance) mutated cells. Our results reveal for the first time a role for small RFC subunits in directing RFC complex functions-in this case towards anti-establishment pathways. We further report that Pds5p exhibits both establishment and anti-establishment functions in cohesion. This duality suggests that categorizations of establishment and anti-establishment activities require further examination.
Mesh Terms:
Acetyltransferases, Cell Cycle Proteins, Chromatids, Chromosomal Proteins, Non-Histone, HSP70 Heat-Shock Proteins, Mitochondrial Proteins, Nuclear Proteins, Protein Subunits, Replication Protein C, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Cell Cycle
Date: Nov. 01, 2010
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