The role of human SWI5-MEI5 complex in homologous recombination repair.
The Swi5-Mei5 complex and its homologues are involved in specialized recombination pathways in budding and fission yeasts. While the fission yeast homologue Swi5-Sfr1 is critical for homologous recombination repair, the budding yeast counterpart Sae3-Mei5 is meiotic specific, interacts with Dmc1 and promotes assembly of Dmc1 on meiotic chromosomes. Here we ... identify and characterize human SWI5-MEI5 (C9orf119-C10orf78) complex. We showed that SWI5 and MEI5 form a stable complex in vitro and in vivo. The C-terminal Swi5 domain of SWI5 and the middle coiled-coil region of MEI5 dictate this conserved interaction. In addition, SWI5-MEI5 directly interacts with RAD51 in vitro. Depletion of SWI5 or MEI5 in human cells causes defects in homologous recombination repair. Finally, SWI5 or MEI5 depleted cells display enhanced sensitivity to ionizing radiation, consistent with the role of this complex in HR repair. Our results suggest that human SWI5-MEI5 has an evolutionarily conserved function in homologous recombination repair.
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Date: Jan. 20, 2011
PubMed ID: 21252223
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