Proteomic identification of the TRAF6 regulation of vacuolar ATPase for osteoclast function.

Osteoclasts are cells specialized for bone resorption. For osteoclast activation, tumor necrosis factor receptor-associated factor 6 (TRAF6) plays a pivotal role. To find new molecules that bind TRAF6 and have a function in osteoclast activation, we employed a proteomic approach. TRAF6-binding proteins were purified from osteoclast cell lysates by affinity ...
chromatography and their identity was disclosed by MS. The identified proteins included several heat shock proteins, actin and actin-binding proteins, and vacuolar ATPase (V-ATPase). V-ATPase, documented for a great increase in expression during osteoclast differentiation, is an important enzyme for osteoclast function; it transports proton to resorption lacunae for hydroxyapatite dissolution. The binding of V-ATPase with TRAF6 was confirmed both in vitro by GST pull-down assays and in osteoclasts by co-immunoprecipitation and confocal microscopy experiments. In addition, the V-ATPase activity associated with TRAF6 increased in osteoclasts stimulated with receptor activator of nuclear factor kappaB ligand (RANKL). Furthermore, a dominant-negative form of TRAF6 abrogated the RANKL stimulation of V-ATPase activity. Our study identified V-ATPase as a TRAF6-binding protein using a proteomics strategy and proved a direct link between these two important molecules for osteoclast function.
Mesh Terms:
Actins, Animals, Bone Marrow Cells, Carrier Proteins, Cells, Cultured, Chromatography, Affinity, Enzyme Activation, Humans, Immunoprecipitation, Membrane Glycoproteins, Mice, Mice, Inbred ICR, Osteoclasts, Protein Binding, Proteomics, RANK Ligand, Receptor Activator of Nuclear Factor-kappa B, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, TNF Receptor-Associated Factor 6, Vacuolar Proton-Translocating ATPases
Proteomics
Date: Nov. 01, 2005
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