SUMOylation of pontin chromatin-remodeling complex reveals a signal integration code in prostate cancer cells.

Posttranslational modification by small ubiquitin-like modifier (SUMO) controls diverse cellular functions of transcription factors and coregulators and participates in various cellular processes including signal transduction and transcriptional regulation. Here, we report that pontin, a component of chromatin-remodeling complexes, is SUMO-modified, and that SUMOylation of pontin is an active control mechanism ...
for the transcriptional regulation of pontin on androgen-receptor target genes in prostate cancer cells. Biochemical purification of pontin-containing complexes revealed the presence of the Ubc9 SUMO-conjugating enzyme that underlies its function as an activator. Intriguingly, 5alpha-dihydroxytestosterone treatments significantly increased the SUMOylation of pontin, and SUMOylated pontin showed further activation of a subset of nuclear receptor-dependent transcription and led to an increase in proliferation and growth of prostate cancer cells. These data clearly define a functional model and provide a link between SUMO modification and prostate cancer progression.
Mesh Terms:
Carrier Proteins, Cell Line, Tumor, Cell Proliferation, Cell Transformation, Neoplastic, Chromatin, DNA Helicases, Humans, Hydroxytestosterones, Lysine, Male, Models, Biological, Prostatic Neoplasms, SUMO-1 Protein, Signal Transduction, Small Ubiquitin-Related Modifier Proteins, Transcription, Genetic, Ubiquitin-Conjugating Enzymes
Proc. Natl. Acad. Sci. U.S.A.
Date: Dec. 26, 2007
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