Estrogen receptor activation function 1 works by binding p160 coactivator proteins.
Estrogen receptor-alpha contains two transactivation functions, a weak constitutive activation function (AF-1) and a hormone-dependent activation function (AF-2). AF-2 works by recruiting a large coactivator complex, composed of one or more p160s, CREB-binding protein (CBP)/p300, and P/CAF (p300 and CBP-associated factor), via direct contacts with the p160s. We report here ... that independent AF-1 activity also requires p160 contacts. Unlike AF-2, which binds signature NR boxes in the center of the p160 molecule, AF-1 binds to sequences near the p160 C terminus. We propose that the ability of AF-1 and AF-2 to interact with separate surfaces of the same coactivator is important for the ability of these transactivation functions to synergize.
Mesh Terms:
Acetyltransferases, Binding Sites, CREB-Binding Protein, Cell Cycle Proteins, Estrogen Receptor alpha, Estrogen Receptor beta, Hela Cells, Histone Acetyltransferases, Humans, Nuclear Proteins, Nuclear Receptor Coactivator 1, Nuclear Receptor Coactivator 2, Nuclear Receptor Coactivator 3, Peptide Fragments, Receptors, Estrogen, Recombinant Proteins, Tamoxifen, Trans-Activators, Transcription Factors, p300-CBP Transcription Factors
Acetyltransferases, Binding Sites, CREB-Binding Protein, Cell Cycle Proteins, Estrogen Receptor alpha, Estrogen Receptor beta, Hela Cells, Histone Acetyltransferases, Humans, Nuclear Proteins, Nuclear Receptor Coactivator 1, Nuclear Receptor Coactivator 2, Nuclear Receptor Coactivator 3, Peptide Fragments, Receptors, Estrogen, Recombinant Proteins, Tamoxifen, Trans-Activators, Transcription Factors, p300-CBP Transcription Factors
Mol. Endocrinol.
Date: Oct. 01, 1998
PubMed ID: 9773983
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