Ganesh K, Adam S, Taylor B, Simpson P, Rada C, Neuberger M

Nuclear proteins typically contain short stretches of basic amino acids (nuclear-localisation sequences; NLSs) which bind karyopherin α family members, directing nuclear import. Here we identify CTNNBL1 (catenin-β-like 1), an armadillo-motif-containing nuclear protein that exhibits no detectable primary sequence homology to karyopherin α, as a novel, selective NLS-binding protein. CTNNBL1 (a ...

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single-copy gene conserved from fission yeast to man) was previously found associated with Prp19-containing RNA splicing complexes as well as with the antibody-diversifying enzyme AID. We find that CTNNBL1 association with the Prp19 complex is mediated by recognition of the NLS of the complex's CDC5L component and show that CTNNBL1 also interacts with Prp31 (another U4/U6.U5 tri-snRNP-associated splicing factor) through its NLS. As with karyopherin αs, CTNNBL1 binds NLSs via its ARM domain, but displays a separate, more selective NLS binding specificity. Furthermore, the CTNNBL1/AID interaction depends on amino acids forming AID's conformational NLS with CTNNBL1-deficient cells showing a partial defect in AID nuclear accumulation. However, in further contrast to karyopherin αs, the CTNNBL1 N-terminal region itself binds karyopherin αs (rather than karyopherin β), suggesting a function divergent from canonical nuclear transport. Thus CTNNBL1 is a novel NLS-binding protein, distinct from karyopherin αs, with the results suggesting a possible role in the selective intranuclear targeting or interactions of some splicing-associated complexes.

Date: Mar. 08, 2011

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