Gcn5 regulates the dissociation of SWI/SNF from chromatin by acetylation of Swi2/Snf2.
The positive link between the SWI/SNF and the Gcn5 histone acetyltransferase in transcriptional activation has been well described. Here we report an inhibitory role for Gcn5 in SWI/SNF targeting. We demonstrate that Gcn5-containing complexes directly acetylate the Snf2 subunit of the SWI/SNF complex in vitro, as well as in vivo. ... Moreover, the acetylation of Snf2 facilitates the dissociation of the SWI/SNF complex from acetylated histones, and reduces its association with promoters in vivo. These data reveal a novel mechanism by which Gcn5 modulates chromatin structure not only through the acetylation of histones, but also by directly acetylating Snf2.
Mesh Terms:
Acetylation, Adenosine Triphosphatases, Chromatin, Fungal Proteins, Histone Acetyltransferases, Histones, Multiprotein Complexes, Promoter Regions, Genetic, Protein Binding, Saccharomyces cerevisiae Proteins, Transcription Factors
Acetylation, Adenosine Triphosphatases, Chromatin, Fungal Proteins, Histone Acetyltransferases, Histones, Multiprotein Complexes, Promoter Regions, Genetic, Protein Binding, Saccharomyces cerevisiae Proteins, Transcription Factors
Genes Dev.
Date: Dec. 15, 2010
PubMed ID: 21159817
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