Characterization of MYR1, a dosage suppressor of YPT6 and RIC1 deficient mutants.
Membrane traffic is tightly regulated and the Rab protein family of small GTPases plays a central role in this regulation. One member of this family is the Saccharomyces cerevisae protein Ypt6. To search for new genes interacting with Ypt6-related pathways, we performed a genetic screen for high copy suppressors of ... ypt6Delta temperature sensitivity at 35 degrees C. Among the suppressors, MYR1 was also able to suppress the temperature sensitive mutant lacking Ric1, a subunit of the Ypt6 guanine exchanging factor complex Ric1/Rgp1. Myr1 is characterized by a coiled coil region and a GYF domain, a protein module binding proline-rich sequences. Myr1 is able to bind membranes but is also associated with larger structures insoluble in Triton X-100. By immunofluorescence, Myr1 shows a network-like pattern as well as small foci. Overexpression of Myr1 influences nuclear envelope morphology and high levels are lethal. This lethality is rescued when the N-terminal region, containing the GYF domain, is deleted. The transcription profile of a myr1Delta strain shows effects on genes involved in nuclear migration, Ras signalling and transcription. Taken together, these results suggest that Myr1 is a novel factor linked to the secretory pathway and important cellular regulatory mechanisms.
Mesh Terms:
Amino Acid Sequence, Carrier Proteins, Cell Membrane, Cell Nucleus Shape, Conserved Sequence, Guanine Nucleotide Exchange Factors, Membrane Proteins, Monomeric GTP-Binding Proteins, Octoxynol, Protein Structure, Tertiary, Protein Transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Deletion, Signal Transduction, Solubility, Suppression, Genetic, Transcription Factors, ras Proteins
Amino Acid Sequence, Carrier Proteins, Cell Membrane, Cell Nucleus Shape, Conserved Sequence, Guanine Nucleotide Exchange Factors, Membrane Proteins, Monomeric GTP-Binding Proteins, Octoxynol, Protein Structure, Tertiary, Protein Transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Deletion, Signal Transduction, Solubility, Suppression, Genetic, Transcription Factors, ras Proteins
Curr. Genet.
Date: Apr. 01, 2008
PubMed ID: 18327588
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