The mammalian immediate-early TIS21 protein and the leukemia-associated BTG1 protein interact with a protein-arginine N-methyltransferase.

The TIS21 immediate-early gene and leukemia-associated BTG1 gene encode proteins with similar sequences. Two-hybrid analysis identified a protein that interacts with TIS21 and BTG1. Sequence motifs associated with S-adenosyl-L-methionine binding suggested this protein might have methyltransferase activity. A glutathione S-transferase (GST) fusion of the putative methyltransferase modifies arginine residues, in ...
appropriate protein substrates, to form NG-monomethyl and NG,NG-dimethylarginine (asymmetric). We term the protein- arginine N-methyltransferase (EC 2.1.1.23) gene "PRMT1, " for protein-arginine methyltransferase 1. GST-TIS21 and GST-BTG1 fusion proteins qualitatively and quantitatively modulate endogenous PRMT1 activity, using control and hypomethylated RAT1 cell extracts as methyl-accepting substrates. PRMT1 message appears ubiquitous, and is constitutive in mitogen-stimulated cells. Modulation of PRMT1 activity by transiently expressed regulatory subunits may be an additional mode of signal transduction following ligand stimulation.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA Primers, DNA, Complementary, Escherichia coli, Genes, Tumor Suppressor, Glutathione Transferase, Humans, Immediate-Early Proteins, Leukemia, Lymphocytic, Chronic, B-Cell, Macromolecular Substances, Mammals, Methyltransferases, Molecular Sequence Data, Neoplasm Proteins, Open Reading Frames, Polymerase Chain Reaction, Protein Biosynthesis, Protein Structure, Secondary, Proteins, Rats, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Sequence Homology, Amino Acid, Tumor Suppressor Proteins
J. Biol. Chem.
Date: Jun. 21, 1996
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