PGC-1 and PERC, coactivators of the estrogen receptor-related receptor gamma.
The mouse nuclear receptor ERRgamma (estrogen receptor-related receptor gamma) is highly expressed in heart, skeletal muscle, kidney, and brain, as well as in the developing nervous system. We found that the expression of the coactivators PGC-1 (PGC-1alpha) and PERC (PGC-1beta) in mammalian cells augmented potently the transcriptional activation by ERRgamma. ... The constitutive activation function 2 (AF-2) of the orphan receptor was important for the synergistic enhancement. Functional receptor truncation analysis revealed an additional amino-terminal activation function, specific for the ERRgamma2 isoform and PGC-1. In vitro experiments showed a direct interaction of ERRgamma with both coactivators. Our findings suggest distinct regulatory functions for PGC-1 and PERC as tissue-specific coactivators for ERRgamma.
Mesh Terms:
3T3 Cells, Animals, Carrier Proteins, Cell Line, Cell Nucleus, Mice, Protein Structure, Tertiary, Receptors, Cytoplasmic and Nuclear, Receptors, Estrogen, Transcription Factors, Transcriptional Activation
3T3 Cells, Animals, Carrier Proteins, Cell Line, Cell Nucleus, Mice, Protein Structure, Tertiary, Receptors, Cytoplasmic and Nuclear, Receptors, Estrogen, Transcription Factors, Transcriptional Activation
Biochem. Biophys. Res. Commun.
Date: Dec. 20, 2002
PubMed ID: 12470660
View in: Pubmed Google Scholar
Download Curated Data For This Publication
11545
Switch View:
- Interactions 6