Papillomavirus binding factor binds to SAP30 and represses transcription via recruitment of the HDAC1 co-repressor complex.

Papillomavirus binding factor, PBF, identical to the Huntington's disease binding protein 2, HDBP2, is a nuclear-cytoplasmic shuttling factor with the ability to inhibit cell growth. It has been identified by its ability to bind to GC-rich sequence elements within upstream promoter regions of certain human papillomavirus (HPV) types and of ...
the Huntingtin protein, respectively. Here, we show that PBF acts as a repressor of HPV transcription. This repression requires the DNA-binding activity of PBF, which we mapped to two C-terminal four-amino acids motifs conserved to the so-called e-tail of certain T-cell factors. Moreover, we show that PBF directly binds to SAP30 (Sin3-associated polypeptide of 30kDa) a component of the mSIN3A-HDAC1 complex, via amino acids 263-312. The addition of Trichostatin A, an inhibitor of HDACs, alleviated PBF-mediated repression. Thus, PBF-mediated repression of transcription involves specific DNA-binding and the recruitment of the SIN3A-HDAC1 complex.
Mesh Terms:
Amino Acid Motifs, Amino Acid Sequence, DNA-Binding Proteins, Hela Cells, Histone Deacetylase 1, Histone Deacetylases, Humans, Hydroxamic Acids, Molecular Sequence Data, Protein Binding, Protein Interaction Mapping, Protein Structure, Tertiary, Sequence Homology, Amino Acid, T-Lymphocytes, Transcription Factors, Transcription, Genetic
Arch. Biochem. Biophys.
Date: Nov. 01, 2007
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