Ikaros DNA-binding proteins direct formation of chromatin remodeling complexes in lymphocytes.
The Ikaros gene family encodes zinc finger DNA-binding proteins essential for lineage determination and control of proliferation in the lymphoid system. Here, we report that, in the nucleus of a T cell, a major fraction of Ikaros and Aiolos proteins associate with the DNA-dependent ATPase Mi-2 and histone deacetylases, in ... a 2 MD complex. This Ikaros-NURD complex is active in chromatin remodeling and histone deacetylation. Upon T cell activation, Ikaros recruits Mi-2/HDAC to regions of heterochromatin. These studies reveal that Ikaros proteins are capable of targeting chromatin remodeling and deacetylation complexes in vivo. We propose that the restructuring of chromatin is a key aspect of Ikaros function in lymphocyte differentiation.
Mesh Terms:
Adenosine Triphosphatases, Animals, Autoantigens, Carrier Proteins, Cell Fractionation, Chromatin, DNA Helicases, DNA-Binding Proteins, Drosophila Proteins, G1 Phase, Histone Deacetylases, Ikaros Transcription Factor, Macromolecular Substances, Mice, Mice, Mutant Strains, Nuclear Proteins, Nucleosomes, S Phase, Spleen, T-Lymphocytes, Trans-Activators, Transcription Factors, Zinc Fingers
Adenosine Triphosphatases, Animals, Autoantigens, Carrier Proteins, Cell Fractionation, Chromatin, DNA Helicases, DNA-Binding Proteins, Drosophila Proteins, G1 Phase, Histone Deacetylases, Ikaros Transcription Factor, Macromolecular Substances, Mice, Mice, Mutant Strains, Nuclear Proteins, Nucleosomes, S Phase, Spleen, T-Lymphocytes, Trans-Activators, Transcription Factors, Zinc Fingers
Immunity
Date: Mar. 01, 1999
PubMed ID: 10204490
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