Yu Y, Srinivasan M, Nakanishi S, Leatherwood J, Shilatifard A, Sternglanz R

A screen of yeast histone alanine substitution mutants revealed that mutations in any of three adjacent residues, L97, Y98 or G99, near the C-terminus of H4 led to a unique phenotype. The mutants grew slowly, became polyploid or aneuploid rapidly and also lost chromosomes at a high rate, most likely ...

Read More

because their kinetochores were not assembled properly. There was lower histone occupancy not only at the centromeric region, but also throughout the genome for the H4 mutants. The mutants displayed genetic interactions with the genes encoding two different histone chaperones, Rtt106 and CAF-I. Affinity purification of Rtt106 and CAF-I from yeast showed that much more H4 and H3 were bound to these histone chaperones in the case of the H4 mutants compared to wild type. However, in vitro binding experiments showed that the H4 mutant proteins bound somewhat more weakly to Rtt106 than did wild type H4. These data suggest that the H4 mutant proteins, along with H3, accumulate on Rtt106 and CAF-I in vivo because they cannot be deposited efficiently on DNA or passed on to the next step in the histone deposition pathway, and this contributes to the observed genome instability and growth defects.

Date: Mar. 28, 2011

View in: Pubmed Google Scholar

116024