Interaction between GRIP and liprin-alpha/SYD2 is required for AMPA receptor targeting.
Interaction with the multi-PDZ protein GRIP is required for the synaptic targeting of AMPA receptors, but the underlying mechanism is unknown. We show that GRIP binds to the liprin-alpha/SYD2 family of proteins that interact with LAR receptor protein tyrosine phosphatases (LAR-RPTPs) and that are implicated in presynaptic development. In neurons, ... liprin-alpha and LAR-RPTP are enriched at synapses and coimmunoprecipitate with GRIP and AMPA receptors. Dominant-negative constructs that interfere with the GRIP-liprin interaction disrupt the surface expression and dendritic clustering of AMPA receptors in cultured neurons. Thus, by mediating the targeting of liprin/GRIP-associated proteins, liprin-alpha is important for postsynaptic as well as presynaptic maturation.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Animals, Brain, COS Cells, Carrier Proteins, Cells, Cultured, Embryo, Mammalian, Nerve Tissue Proteins, Neurons, Phosphoproteins, Protein Tyrosine Phosphatases, Rats, Receptor-Like Protein Tyrosine Phosphatases, Class 2, Receptors, AMPA, Receptors, Cell Surface, Synapses
Adaptor Proteins, Signal Transducing, Animals, Brain, COS Cells, Carrier Proteins, Cells, Cultured, Embryo, Mammalian, Nerve Tissue Proteins, Neurons, Phosphoproteins, Protein Tyrosine Phosphatases, Rats, Receptor-Like Protein Tyrosine Phosphatases, Class 2, Receptors, AMPA, Receptors, Cell Surface, Synapses
Neuron
Date: Mar. 28, 2002
PubMed ID: 11931740
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